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naïve cd4 t cell isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec naïve cd4 t cell isolation kit
    Naïve Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4/Naive+CD4%2B+T+Cell+Isolation+Kit%2C+mouse/pmc13427790-70-17-25
    Average 97 stars, based on 432 article reviews
    naïve cd4 t cell isolation kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Targeting P2RY2 reprograms tumor immunity and inhibits tumor growth in non-small cell lung cancer.
    Article Snippet: .. In the second reaction, cells were stained with antibodies against CD45 (clone 30-F11, PerCP), CD8⍺ (PE-Vio770, clone REA601, #130-118-946), CD4 (FITC, clone REA604, #130-118-692), CD3 (APC-Vio770, clone REA641, #130-119-793), CD45R (B220, Vioblue, clone REA755, #130-110-851) and NK1.1 (APC, clone REA1162, #130-120-507), all are from Miltenyi Biotec, except for the CD206, which is from Invitrogen. ..

    Article Title: Epigenetic editing balances TCR suppression and persistence in CAR T cells
    Article Snippet: Using density centrifugation with Pancoll solution (1,077 g/mL) (PanBiotech, Aidenbach, Germany), primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) using a pan T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). .. Isolated T cells were stained with fluorescently labeled mAbs against human CD3 (#130-113-138), CD4 (#130-113-225), CD8 (#130-110-683) (Miltenyi Biotec). ..

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Article Title: Early-Stage B-cells Predict Relapse After Rituximab Treatment in Patients With Membranous Nephropathy
    Article Snippet: .. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). .. The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric).

    Article Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
    Article Snippet: .. After washing, the cells were resuspended in Dulbecco’s phosphate-buffered saline (HyClone, Logan, UT, USA) and stained with fluorochrome-conjugated monoclonal antibodies against human CD3, CD4, and CD8 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). .. The stained cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

    Generated:

    Article Title: LRRC15-CAR T cells for the treatment of osteosarcoma
    Article Snippet: .. Lentiviral transduced T cells were generated as 100 previously described.42 Briefly, CD4+ and CD8+ T cells were enriched from PBMCs by 101 immunomagnetic separation using CD4 (Miltenyi, Cat# 130-045-101, RRID:AB_2889919) and 102 CD8 microbeads (Miltenyi, Cat# 130-045-201, RRID:AB_2889920), an LS column (Miltenyi), 103 and a MidiMACS Separator (Miltenyi) or using an automated system (autoMACs, Miltenyi). .. On 104 D ow nloaded from http://aacrjournals.org/clincancerres/article-pdf/doi/10.1158/1078-0432.C C R -24-2335/3732320/ccr-24-2335.pdf by guest on 09 February 2026 8 Day 0, enriched T cells were resuspended at 1x106 cells/ml in RPMI (GE Healthcare Life 105 Sciences) supplemented with 10% FBS (GE Healthcare Life Sciences), 1% GlutaMAX (Thermo 106 Fisher Scientific), and cytokines IL-7 and IL-15 (10ng/ml each; Biological Resources Branch, 107 National Cancer Institute, Frederick, MD, and Peprotech, Rocky Hill, NJ), and stimulated 108 overnight on 24-well non-tissue culture treated plates that were precoated with CD3 and CD28 109 antibodies (Miltenyi).

    Immunomagnetic Separation:

    Article Title: LRRC15-CAR T cells for the treatment of osteosarcoma
    Article Snippet: .. Lentiviral transduced T cells were generated as 100 previously described.42 Briefly, CD4+ and CD8+ T cells were enriched from PBMCs by 101 immunomagnetic separation using CD4 (Miltenyi, Cat# 130-045-101, RRID:AB_2889919) and 102 CD8 microbeads (Miltenyi, Cat# 130-045-201, RRID:AB_2889920), an LS column (Miltenyi), 103 and a MidiMACS Separator (Miltenyi) or using an automated system (autoMACs, Miltenyi). .. On 104 D ow nloaded from http://aacrjournals.org/clincancerres/article-pdf/doi/10.1158/1078-0432.C C R -24-2335/3732320/ccr-24-2335.pdf by guest on 09 February 2026 8 Day 0, enriched T cells were resuspended at 1x106 cells/ml in RPMI (GE Healthcare Life 105 Sciences) supplemented with 10% FBS (GE Healthcare Life Sciences), 1% GlutaMAX (Thermo 106 Fisher Scientific), and cytokines IL-7 and IL-15 (10ng/ml each; Biological Resources Branch, 107 National Cancer Institute, Frederick, MD, and Peprotech, Rocky Hill, NJ), and stimulated 108 overnight on 24-well non-tissue culture treated plates that were precoated with CD3 and CD28 109 antibodies (Miltenyi).

    Article Title: LRRC15-CAR T Cells for the Treatment of Osteosarcoma
    Article Snippet: .. Briefly, CD4 + and CD8 + T cells were enriched from PBMC by immunomagnetic separation using CD4 (Miltenyi Biotec, cat. #130-045-101, RRID: AB_2889919) and CD8 microbeads (Miltenyi Biotec, cat. #130-045-201, RRID: AB_2889920), an LS column (Miltenyi Biotec), and a MidiMACS Separator (Miltenyi Biotec) or using an automated system (autoMACS, Miltenyi Biotec). .. On day 0, enriched T cells were resuspended at 1 × 10 6 cells/mL in RPMI (GE HealthCare Life Sciences) supplemented with 10% fetal bovine serum (FBS, GE HealthCare Life Sciences), 1% GlutaMAX (Thermo Fisher Scientific), and cytokines IL7 and IL15 (10 ng/mL each; Biological Resources Branch, NCI, and Peprotech) and stimulated overnight on 24-well non-tissue culture–treated plates that were precoated with CD3 and CD28 antibodies (Miltenyi Biotec).

    Isolation:

    Article Title: Epigenetic editing balances TCR suppression and persistence in CAR T cells
    Article Snippet: Using density centrifugation with Pancoll solution (1,077 g/mL) (PanBiotech, Aidenbach, Germany), primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) using a pan T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). .. Isolated T cells were stained with fluorescently labeled mAbs against human CD3 (#130-113-138), CD4 (#130-113-225), CD8 (#130-110-683) (Miltenyi Biotec). ..

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Article Title: Phenotypically and functionally unique CD86 expression CD8 T cell subset shapes immune regulation in the tumor microenvironment.
    Article Snippet: The coculture was maintained for 48 h. On Day 9, OT-1 or OT-II T cell proliferation was assessed using Carboxyfluorescein Succinimidyl Ester (CFSE, Thermo Fisher Scientific) and Ki-67 staining, and cytokine production (IL-2 and IFN-c) was assessed by FCM. .. Cell preparation and cell sorting Popliteal lymph node CD86high /CD86low CD8+ T cells: T cells were isolated from the spleens of OT-I and OT-II donor mice using CD4 (130-104-454; Miltenyi Biotec) and CD8 negative-selection kits. ..

    Labeling:

    Article Title: Epigenetic editing balances TCR suppression and persistence in CAR T cells
    Article Snippet: Using density centrifugation with Pancoll solution (1,077 g/mL) (PanBiotech, Aidenbach, Germany), primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) using a pan T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). .. Isolated T cells were stained with fluorescently labeled mAbs against human CD3 (#130-113-138), CD4 (#130-113-225), CD8 (#130-110-683) (Miltenyi Biotec). ..

    Activation Assay:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    Expressing:

    Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance
    Article Snippet: For analysis of TCE-binding to CD3 + splenocytes isolated from BALB/c mice, cells were stained with FITC-coupled anti-mouse-CD3 antibody (Miltenyi Biotec #130–119-758). .. For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461). ..

    FACS:

    Article Title: Phenotypically and functionally unique CD86 expression CD8 T cell subset shapes immune regulation in the tumor microenvironment.
    Article Snippet: The coculture was maintained for 48 h. On Day 9, OT-1 or OT-II T cell proliferation was assessed using Carboxyfluorescein Succinimidyl Ester (CFSE, Thermo Fisher Scientific) and Ki-67 staining, and cytokine production (IL-2 and IFN-c) was assessed by FCM. .. Cell preparation and cell sorting Popliteal lymph node CD86high /CD86low CD8+ T cells: T cells were isolated from the spleens of OT-I and OT-II donor mice using CD4 (130-104-454; Miltenyi Biotec) and CD8 negative-selection kits. ..

    Bioprocessing:

    Article Title: Early-Stage B-cells Predict Relapse After Rituximab Treatment in Patients With Membranous Nephropathy
    Article Snippet: .. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). .. The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric).

    Article Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
    Article Snippet: .. After washing, the cells were resuspended in Dulbecco’s phosphate-buffered saline (HyClone, Logan, UT, USA) and stained with fluorochrome-conjugated monoclonal antibodies against human CD3, CD4, and CD8 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). .. The stained cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

    Saline:

    Article Title: Establishment of a serum-free culture system with an optimized 5-interleukin cytokine cocktail and insulin to promote preferential CD8⁺ T-cell expansion.
    Article Snippet: .. After washing, the cells were resuspended in Dulbecco’s phosphate-buffered saline (HyClone, Logan, UT, USA) and stained with fluorochrome-conjugated monoclonal antibodies against human CD3, CD4, and CD8 (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). .. The stained cells were analyzed using a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).



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    Image Search Results


    The ZC3H13/CCND1 axis remodels the HNSCC immune microenvironment and the proposed mechanistic model. (A) Gene Ontology (GO) enrichment analysis revealing biological pathways related to the negative regulation of cell activation and apoptosis. (B) Bar plot illustrating the changes in the proportion of CD4 + T cells between the Pre‐ and Post‐immunotherapy groups. (C) Boxplot comparing the quantitative CytoTRACE scores before and after immunotherapy (Pre vs. Post). (D) UMAP feature plots displaying the spatial distribution of cell differentiation states (CytoTRACE scores) in Pre‐ and Post‐treatment samples. (E) Violin plot demonstrating the significant difference in CD4+ T cell exhaustion scores between the Pre and Post treatment groups. (F) The relative mRNA expression levels of key immune checkpoint molecules (PDCD1, LAG3, CTLA4) in CCND1‐low and CCND1‐high groups were determined by RT‐qPCR. (G) Representative multiplex immunofluorescence images showing CCND1, CD4, PD‐1 and DAPI staining in mouse HNSCC tissues from control and ZC3H13‐deficient groups. Scale bar, 20 µm. (H) The quantification for the multiplex immunofluorescence (mIF) staining in the tumour microenvironment. (I) Schematic mechanistic model. Data are presented as the mean ± SD. *** p < .001.

    Journal: Clinical and Translational Medicine

    Article Title: ZC3H13‐mediated m6A stabilization of CCND1 promotes malignant progression and is associated with poor anti‐PD‐1 response in HNSCC

    doi: 10.1002/ctm2.70750

    Figure Lengend Snippet: The ZC3H13/CCND1 axis remodels the HNSCC immune microenvironment and the proposed mechanistic model. (A) Gene Ontology (GO) enrichment analysis revealing biological pathways related to the negative regulation of cell activation and apoptosis. (B) Bar plot illustrating the changes in the proportion of CD4 + T cells between the Pre‐ and Post‐immunotherapy groups. (C) Boxplot comparing the quantitative CytoTRACE scores before and after immunotherapy (Pre vs. Post). (D) UMAP feature plots displaying the spatial distribution of cell differentiation states (CytoTRACE scores) in Pre‐ and Post‐treatment samples. (E) Violin plot demonstrating the significant difference in CD4+ T cell exhaustion scores between the Pre and Post treatment groups. (F) The relative mRNA expression levels of key immune checkpoint molecules (PDCD1, LAG3, CTLA4) in CCND1‐low and CCND1‐high groups were determined by RT‐qPCR. (G) Representative multiplex immunofluorescence images showing CCND1, CD4, PD‐1 and DAPI staining in mouse HNSCC tissues from control and ZC3H13‐deficient groups. Scale bar, 20 µm. (H) The quantification for the multiplex immunofluorescence (mIF) staining in the tumour microenvironment. (I) Schematic mechanistic model. Data are presented as the mean ± SD. *** p < .001.

    Article Snippet: Sections were incubated with the indicated primary antibodies at room temperature for 1 h: CD4 (HY‐ P83756 , MCE, 1:500), CCND1 (HY‐ P80098 , MCE, 1:50), and PD‐1 (84651, Cell Signaling Technology, 1:100) in the optimized order of CD4, CCND1, and PD‐1.

    Techniques: Activation Assay, Cell Differentiation, Expressing, Quantitative RT-PCR, Multiplex Assay, Immunofluorescence, Staining, Control